Ubuchwepheshe be-Ultrafiltration ubuchwepheshe bokuhlukanisa obusha nobusezingeni eliphezulu.Inezici zenqubo elula, inzuzo ephezulu yezomnotho, akukho ukuguqulwa kwesigaba, i-coefficient yokuhlukanisa enkulu, ukonga amandla, ukusebenza kahle okuphezulu, akukho ukungcola kwesibili, ukusebenza okuqhubekayo ekamelweni lokushisa njalonjalo.Namuhla, umphathi u-Yang ovela e-Beijing wabuza mayelana nemishini yethu yokuhluza amaprotheni futhi waxhumana ngokuningiliziwe ngobuchwepheshe bethu.Manje, umhleli weqembu leShandong Bona uzokwethula ukusetshenziswa kwe-ultrafiltration ekuhlukaniseni amaprotheni kanye nokuhlanzwa.
1. Ukuze uthole amaprotheni desalination, dealcoholization kanye nokugxila
Izicelo ezibaluleke kakhulu ze-ultrafiltration ekuhlanzweni kwamaprotheni ukukhipha usawoti nokugxila.Indlela ye-Ultrafiltration yokukhipha usawoti kanye nokugxila ibonakala ngevolumu ye-batch enkulu, isikhathi esifushane sokusebenza kanye nokusebenza kahle okuphezulu kokubuyiswa kwamaprotheni.Indlela yendabuko ye-steric exclusion chromatography yokususa izinto ezahlukahlukene kumaprotheni ithathelwe indawo ubuchwepheshe besimanje be-ultrafiltration, obube ubuchwepheshe obuyinhloko bokukhipha usawoti emanzini, ukudakwa kanye nokugxilisa ingqondo namuhla.Eminyakeni yamuva nje, ubuchwepheshe be-ultrafiltration buye basetshenziswa kabanzi ekukhishweni kukasawoti emanzini nasekubuyiseni amaprotheni anenani eliphezulu lokudla okunempilo ku-cheese whey kanye ne-soya whey.I-Lactose nosawoti kanye nezinye izingxenye zeprotheni, kanye nezidingo zangempela zokuqeda ngempumelelo ukukhishwa kwe-desalting, de-alcoholization kanye nokugxila kwamaprotheni.Ukusetshenziswa kobuchwepheshe be-ultrafiltration kungaphinda kugxilise ama-serospecies immunoglobulins ukuze kuhlangatshezwane nesidingo sangempela sesivuno samaprotheni.
2. Ngokwahlukaniswa kwamaprotheni
Ukuhlukaniswa kwamaprotheni kubhekisela enqubweni yokuhlukanisa ingxenye ngayinye yeprotheni ngesigaba ngokuya ngomehluko wezakhiwo zomzimba namakhemikhali (njengesisindo sengqamuzana elihlobene, iphoyinti le-isoelectric, i-hydrophobicity, njll.) yengxenye ngayinye yeprotheni oketshezini lokuphakelayo.I-Gel chromatography ingenye yezindlela ezivame ukusetshenziswa kakhulu zokuhlukaniswa kwama-macromolecule ebhayoloji (ikakhulukazi amaprotheni).Uma kuqhathaniswa ne-chromatography evamile, ubuchwepheshe bokuhlukaniswa kwe-ultrafiltration bunethemba elihle lokusetshenziswa ekuhlukaniseni nasekukhiqizeni izimboni zamaprotheni nama-enzyme anenani elibalulekile lezomnotho ngenxa yezindleko eziphansi kanye nokukhulisa kalula.Iqanda elimhlophe liyinto eluhlaza eshibhe kakhulu yokuthola i-lysozyme ne-ovalbumin.Muva nje, i-ultrafiltration ivame ukusetshenziswa ukuhlukanisa i-ovalbumin ne-lysozyme kumhlophe kweqanda.
3. Ukususwa kwe-endotoxin
Ukususwa kwe-endotoxin kungenye yezindlela eziyinhloko zokusetshenziswa kobuchwepheshe be-ultrafiltration ekuhlanzeni amaprotheni.Inqubo yokukhiqiza ye-endotoxin iyinkimbinkimbi kakhulu.Enqubweni yokusetshenziswa okungokoqobo, ngoba iphrotheni yokwelapha ekhiqizwa uhlelo lwe-prokaryotic expression kulula ukuxutshwa ne-endotoxin ekhiqizwa ukuphuka kwe-cell cell yamagciwane, futhi i-endotoxin, eyaziwa nangokuthi i-pyrogen, iwuhlobo lwe-lipopolysaccharide.Ngemva kokungena emzimbeni womuntu, kungabangela umkhuhlane, ukuphazamiseka kwe-microcirculation, ukushaqeka kwe-endotoxic nezinye izimpawu.Ukuze uvikele impilo yomuntu, kuyadingeka ukusebenzisa ubuchwepheshe be-ultrafiltration ngokugcwele ukuze kukhishwe ama-endotoxins.
Nakuba ubuchwepheshe be-ultrafiltration busetshenziswa kabanzi ekuhlukaniseni nasekuhlanzeni amaprotheni, bubuye bube nemikhawulo ethile.Uma isisindo samangqamuzana semikhiqizo emibili ezohlukaniswa singaphansi kwezikhathi ezi-5, asikwazi ukuhlukaniswa nge-ultrafiltration.Uma isisindo se-molecular yomkhiqizo singaphansi kuka-3kD, asikwazi ukugxiliswa ngokucwenga okukhulu, ngoba ukuhlunga kwe-ultrafilt ngokuvamile kwenziwa ngesisindo esincane se-molecular of the membrane ku-1000 NWML.
Ngokuthuthuka okuqhubekayo kwemboni ye-bioengineering, izidingo eziphakeme zibekwe phambili zokuhlukaniswa komfula kanye nobuchwepheshe bokuhlanza.Izindlela zendabuko zokugxilisa i-vacuum, ukukhipha i-solvent, i-dialysis, i-centrifugation, imvula kanye nokususwa kwe-pyrogen azisatholakali ukuhlangabezana nezidingo zokukhiqiza.Ubuchwepheshe be-Ultrafiltration nakanjani buzosetshenziswa kabanzi ngoba izinzuzo zabo ekuhlukaniseni amaprotheni.
Isikhathi sokuthumela: Apr-20-2022